Yeast (Saccharomyces cerevisiae) GC/EI/MS metabolomics dataset

Area of Science:

• Metabolomics
• Yeast Genetics
• Fungicide Resistance

Background:

• The ABC transporter YCF1 is located in the yeast vacuole membrane.
• Understanding fungicide resistance mechanisms in Saccharomyces cerevisiae is crucial for agricultural applications.
• Yeast serves as a model organism for studying cellular transport and metabolism.

Purpose of the Study:

• To globally profile the endo-metabolome of wild and genetically engineered yeast strains.
• To investigate the involvement of the ABC transporter YCF1 in resistance to the fungicide flusilazole.
• To provide a reference dataset for yeast metabolomics studies.

Main Methods:

• Gas chromatography-electron impact-mass spectrometry (GC/EI/MS) was employed for global metabolomic profiling.
• Wild and genetically engineered Saccharomyces cerevisiae strains were treated with sub-lethal doses of flusilazole.
• Raw GC/EI/MS total ion chromatograms were recorded and are included as a dataset.

Main Results:

• Metabolomic profiling revealed changes in the yeast endo-metabolome upon flusilazole treatment.
• The study provides evidence for the implication of the YCF1 transporter in yeast resistance to flusilazole.
• The dataset serves as a valuable resource for comparative metabolomics.

Conclusions:

• The YCF1 vacuole transporter plays a significant role in Saccharomyces cerevisiae resistance to the fungicide flusilazole.
• GC/EI/MS metabolomics is a powerful approach for dissecting mechanisms of xenobiotic resistance in yeast.
• The generated dataset supports further research into yeast metabolism and bioactive compound interactions.