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Smartphone DNA or RNA Sensing Using Semisynthetic Luciferase-Based Logic Device
Dalu Chang1, Ki Tae Kim1, Eric Lindberg1
1Department of Organic Chemistry, NCCR Chemical Biology, Faculty of Science, University of Geneva, 30 quai Ernest Ansermet, 1211 Geneva, Switzerland.
ACS Sensors
|March 4, 2020
Summary
We developed a novel bioluminescent sensor using a semisynthetic luciferase and smartphone quantification for detecting specific oligonucleotide sequences. This technology enables sensitive microRNA detection for point-of-care diagnostics.
Area of Science:
- Biochemistry
- Molecular Biology
- Biotechnology
Background:
- Oligonucleotide detection is crucial for diagnostics.
- Existing methods often lack point-of-care accessibility.
- Need for sensitive and user-friendly detection technologies.
Purpose of the Study:
- To develop a novel bioluminescent sensor for specific oligonucleotide sequence detection.
- To enable smartphone-based quantification for point-of-care applications.
- To demonstrate the sensor's capability in detecting cancer biomarker microRNAs.
Main Methods:
- Utilized a semisynthetic luciferase (H-Luc-PNA conjugate) as the core sensor component.
- Employed a strand-displacement reaction to activate the bioluminescent signal.
- Integrated smartphone quantification for readout.
- Demonstrated sensing of three distinct microRNAs (miRs).
- Implemented an AND gate for simultaneous detection of two sequences.
Main Results:
- Successfully developed a bioluminescent sensor with smartphone quantification.
- Achieved sensitive detection of three different microRNAs (miRs).
- Demonstrated the feasibility of a dual-sequence detection AND gate.
- The H-Luc-PNA conjugate showed reliable performance in strand-displacement reactions.
Conclusions:
- The developed bioluminescent sensor technology is suitable for specific oligonucleotide detection.
- Smartphone quantification offers a viable approach for point-of-care diagnostics.
- The sensor platform shows promise for multiplexed detection of cancer biomarkers.
