Related Experiment Video

Updated: Dec 27, 2025

Introduction to Solid Supported Membrane Based Electrophysiology
19:56

Introduction to Solid Supported Membrane Based Electrophysiology

Published on: May 11, 2013

15.6K

Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a

Gabriel García-Molina1, Paolo Natale2, Laura Valenzuela3

  • 1Instituto de Catálisis y Petroleoquímica, CSIC, c/Marie Curie 2, 28049 Madrid, Spain.

Bioelectrochemistry (Amsterdam, Netherlands)
|March 4, 2020
PubMed

Keywords:
ATPATP-synthaseBiomimetic membranePotentiometric biosensor

More Related Videos

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.5K
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy12:15

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

Published on: February 21, 2019

7.8K

Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
1.5K
Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential...
572
ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
9.5K
Potentiometry: Overview01:06

Potentiometry: Overview

Potentiometry is an analytical technique that measures the potential difference between two electrodes in an electrochemical cell without drawing any significant current that could alter the solution's composition. This method employs an indicator electrode, which exchanges electrons with the analyte solution, and a reference electrode with a constant potential. Each electrode is immersed in a solution comprised of two half-cells. In a conventional setup, the reference electrode serves as...
4.0K
Amperometry: Overview01:10

Amperometry: Overview

Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
1.5K
Potentiometric Titration: Overview01:31

Potentiometric Titration: Overview

Potentiometric titration is a quantitative analytical technique that determines the concentration of an analyte by measuring the potential difference between the two electrodes in the solution. The endpoint of a potentiometric titration is the point at which there is a significant change in the potential difference. It occurs when the stoichiometric reaction between the analyte and the titrant is complete. The endpoint is usually determined graphically by plotting the measured potential...
3.8K