Improving yield-related physiological characteristics of spring rapeseed by integrated fertilizer management under...

Two separate field experiments were conducted in 2018 and 2019 as split-plot based on randomized complete block design with three replications to evaluate physiological responses of rapeseed to fertilization treatments (control, chemical fertilizer, inoculation of seeds with PGPR, vermicompost and combined fertilizers) under different irrigation levels (irrigation after 70,100, 130, and 160 mm evaporation). Water stress increased the activities of catalase, polyphenol oxidase, peroxidase and superoxide dismutase and the contents of proline, soluble sugars and malondialdehyde and also leaf temperature, but decreased membrane stability index, chlorophyll content, leaf water content, stomatal conductance and grain yield. Application of fertilizers particularly combined fertilizers decreased proline content and leaf temperature, but increased the antioxidant enzymes activities, soluble sugars, chlorophyll content, leaf water content, membrane stability index, and stomatal conductance under different irrigation intervals. These superiorities of fertilization treatments were led to considerable improvement in grain yield. The results revealed that the combined fertilizer application improved most of the physiological parameters. It was deducted that the application of combined fertilizers reduced chemical fertilizer by about 67% and alleviated the deleterious effects of water limitation on field performance of rapeseed.