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The effect of isatin on the Echinococcus granulosus cyst in an experimental host
M A Piens1, E Sarciron, P Audin
1Departement de Parasitologie et Pathologie Exotique, Université Claude Bernard, Lyon, France.
Abstract:
The effect of isatin on the E. granulosus cyst was studied. NMRI mice, which were infected with E. granulosus of sheep origin, were treated daily with isatin at a dose of 50 mg kg-1 for 18 days. Ultrastructural damage was observed in the treated cysts, including accumulation of lamellar stacks, electron-dense granules, autophagosomes and lipid vesicles. Moreover, a biochemical study showed an inhibition of alkaline phosphatase activity, with a decrease in carbohydrate storage and an increase in acid phosphatase activity. In spite of the short duration of the treatment, the results obtained allowed us to conclude that isatin acts on E. granulosus cysts. This activity appears as a process of degeneration linked to the alkaline phosphatase inhibitory effect of isatin.
Insights
Isatin treatment caused significant ultrastructural damage to Echinococcus granulosus cysts in mice. This parasite degeneration was linked to altered enzyme activity and reduced carbohydrate storage.
Area of Science:
- Parasitology
- Biochemistry
- Cell Biology
Background:
- Echinococcus granulosus is a parasitic cestode causing cystic echinococcosis.
- Current treatments for cystic echinococcainfection have limitations.
- Isatin is a compound with potential biological activities.
Purpose of the Study:
- To investigate the effects of isatin on Echinococcus granulosus cysts.
- To evaluate the ultrastructural and biochemical changes induced by isatin.
Main Methods:
- NMRI mice infected with E. granulosus were treated daily with isatin (50 mg/kg) for 18 days.
- Ultrastructural analysis of cysts was performed using electron microscopy.
- Biochemical assays measured alkaline phosphatase and acid phosphatase activity, and carbohydrate storage.
Main Results:
- Isatin treatment induced significant ultrastructural damage in E. granulosus cysts.
- Observed damage included lamellar stacks, electron-dense granules, autophagosomes, and lipid vesicles.
- Biochemical analysis revealed inhibition of alkaline phosphatase, decreased carbohydrate storage, and increased acid phosphatase activity.
Conclusions:
- Isatin demonstrates a degenerative effect on E. granulosus cysts.
- The observed activity is likely related to the inhibition of alkaline phosphatase by isatin.
- Isatin shows potential as a therapeutic agent against cystic echinococcosis.