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Published on: June 12, 2010
Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings
A Ya Bolsunovsky1, D V Dementyev2, T S Frolova3,4
1Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, 660036, Krasnoyarsk, Russia. radecol@ibp.ru.
Abstract:
The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.
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