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Related Experiment Video

Updated: Dec 27, 2025

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NUDT7 Loss Promotes Kras CRC Development.

Jinsoo Song1,2, Sujeong Park1, Jinjoo Oh1

  • 1Department of Biological Sciences, College of Natural Sciences, Wonkwang University, Iksan, Chunbuk 54538, Korea.

Cancers
|March 6, 2020
PubMed
Summary

Peroxisomal NUDT7 is crucial in Kras-driven colorectal cancer (CRC). Reduced NUDT7 levels promote lipid accumulation and CRC gene expression, while its restoration has protective effects, highlighting NUDT7 as a therapeutic target.

Keywords:
colorectal cancerpalmitic acidperoxisomal coenzyme A diphosphatase NUDT7 (NUDT7)peroxisomeβ-catenin

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Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Dysregulation of peroxisomal lipid metabolism is implicated in colorectal cancer (CRC) pathogenesis.
  • Kras-driven CRC tumors exhibit impaired peroxisomal beta-oxidation.

Purpose of the Study:

  • To investigate the role of Nudt7 (peroxisomal coenzyme A diphosphatase NUDT7) in Kras-driven colorectal cancer.
  • To elucidate the molecular mechanisms linking Nudt7 dysfunction to CRC development.

Main Methods:

  • Utilized Nudt7 knockout mice and Nudt7-overexpressing cell lines in Kras-driven CRC models.
  • Administered azoxymethane/dextran sulfate sodium (AOM/DSS) to induce colitis-associated cancer.
  • Performed microarray analysis and Ingenuity Pathway Analysis (IPA).
  • Assessed lipid accumulation, gene expression (CRC-related, Wnt signaling pathway components), and beta-catenin levels.

Main Results:

  • Nudt7 expression was significantly decreased in Kras-driven CRC tumors.
  • Nudt7 deficiency led to increased lipid accumulation and CRC-related gene expression.
  • Nudt7 overexpression reduced lipid accumulation and CRC gene expression.
  • Wnt signaling pathway activation, indicated by upregulated beta-catenin and its targets (Myc, Ccdn1, Nos2), was observed in Nudt7-deficient colons.
  • Elevated palmitic acid levels in Nudt7-deficient colons correlated with induced beta-catenin and CRC gene expression.

Conclusions:

  • Peroxisomal NUDT7 plays a critical role in suppressing Kras-driven colorectal cancer development.
  • Nudt7 deficiency promotes CRC by disrupting lipid metabolism and activating Wnt/beta-catenin signaling.
  • NUDT7 represents a potential therapeutic target for colorectal cancer.