Inhibition of Phytosterol Biosynthesis by Azasterols
Sylvain Darnet1, Laetitia B B Martin2, Pierre Mercier2
1CVACBA, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém 66075-750, Brazil.
Abstract:
Inhibitors of enzymes in essential cellular pathways are potent probes to decipher intricate physiological functions of biomolecules. The analysis of Arabidopsis thaliana sterol profiles upon treatment with a series of azasterols reveals a specific in vivo inhibition of SMT2, a plant sterol-C-methyltransferase acting as a branch point between the campesterol and sitosterol biosynthetic segments in the pathway. Side chain azasteroids that modify sitosterol homeostasis help to refine its particular function in plant development.
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