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Silencing Delta-like 1 Expression Induces Migratory Features in Pancreatic Cancer Cells Through Stimulation of Src

Jungwhoi Lee1, Jungsul Lee2, DA Hye Kim3

  • 1Department of Applied Life Science, SARI, Jeju National University, Jeju-do, Republic of Korea sdjd1108@kaist.ac.kr kimjh@jejunu.ac.kr.

Anticancer Research
|March 6, 2020
PubMed
Abstract

Insights

Delta-like 1 (DLL1) suppresses pancreatic cancer metastasis by inhibiting cell migration and invasion. High DLL1 expression correlates with a better prognosis in pancreatic cancer patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Pancreatic cancer prognosis remains poor due to metastasis and chemo-resistance, with unclear underlying mechanisms.
  • Delta-like 1 (DLL1) is investigated for its role in the metastatic potential of human pancreatic cancer cells.

Purpose of the Study:

  • To investigate the function of delta-like 1 (DLL1) as a tumor suppressor in human pancreatic cancer cell metastasis.

Main Methods:

  • Analyzed DLL1 expression using GEO public database and western blot.
  • Validated DLL1's biological function through cell behavior analysis.
  • Correlated DLL1 expression with patient prognosis using the GEO public database.

Main Results:

  • DLL1 mRNA and protein were upregulated in pancreatic cancer tissues and cell lines.
  • DLL1 ablation enhanced migration and invasion by activating Src and p38 phosphorylation.
  • DLL1 expression correlated with reduced in vivo metastasis and a favorable patient prognosis.

Conclusions:

  • DLL1 acts as a potent suppressor of pancreatic cancer metastasis.
  • Understanding DLL1's expression-function correlation advances knowledge of pancreatic cancer metastasis mechanisms.
Keywords:
DLL1Srcmetastasisp38pancreatic cancersuppressor

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