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Related Concept Videos

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RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Updated: Dec 27, 2025

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
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Clonal tracking using embedded viral barcoding and high-throughput sequencing.

Charles Bramlett1, Du Jiang1, Anna Nogalska1

  • 1Eli and Edythe Broad CIRM Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California, Los Angeles, Los Angeles, CA, USA.

Nature Protocols
|March 6, 2020
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Summary
This summary is machine-generated.

Viral barcoding with high-throughput sequencing offers a cost-effective method to track single-cell clones. This protocol details a procedure for precise quantification of cellular progeny in various experimental settings.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • Single-cell clone tracking is crucial for understanding cellular dynamics.
  • Existing methods may lack sensitivity, throughput, or cost-efficiency.

Purpose of the Study:

  • To present a detailed protocol for viral barcoding and high-throughput sequencing.
  • To enable clonal-level insights into cellular processes and treatment efficacy.

Main Methods:

  • Creation of barcode libraries.
  • Viral delivery and recovery of barcodes.
  • Computational analysis of sequencing data.

Main Results:

  • The entire procedure can be completed within weeks.
  • High sensitivity, throughput, and precise quantification of cellular progeny.
  • Cost-efficient and requires no advanced skills.

Conclusions:

  • Viral barcoding is a powerful, adaptable technology for tracking single-cell clones.
  • Suitable for both in vitro and in vivo experiments.
  • Provides clonal-level insights into proliferation, differentiation, and treatment response.