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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
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Related Experiment Video

Updated: Dec 27, 2025

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Thermal proteome profiling for interrogating protein interactions.

André Mateus¹, Nils Kurzawa^1,2, Isabelle Becher¹

¹Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Molecular Systems Biology

|March 6, 2020

Summary

This summary is machine-generated.

Thermal proteome profiling (TPP) measures changes in protein stability using mass spectrometry. This technique reveals protein interactions and drug targets in various biological contexts.

Keywords:

drug discovery metabolites protein complexes proteomics thermal proteome profiling

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Area of Science:

Biochemistry
Proteomics
Chemical Biology

Background:

Proteins undergo denaturation and insolubilization when exposed to heat.
Protein thermal stability is altered by interactions with small molecules, nucleic acids, proteins, and post-translational modifications.

Purpose of the Study:

To present Thermal Proteome Profiling (TPP) as a method for studying protein interactions and drug targets.
To highlight the versatility of TPP across in vitro, in situ, and in vivo systems.

Main Methods:

Utilizes multiplexed quantitative mass spectrometry-based proteomics.
Monitors the melting profiles of thousands of proteins simultaneously.
Assesses changes in protein thermal stability.

Main Results:

Successfully applied to identify drug targets and off-targets.
Enabled the study of protein-metabolite and protein-protein interactions.
Provides insights into protein state and interactions within their native context.

Conclusions:

TPP offers a unique, proteome-wide view of protein behavior.
Facilitates the study of fundamental biological processes and their mechanisms.
A powerful tool for drug discovery and understanding cellular mechanisms.