Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.8K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

697
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
697
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Network Pharmacology Study on the Active Ingredients and Potential Targets of <i>Tripterygium wilfordii</i> Hook for Treatment of Rheumatoid Arthritis.

Evidence-based complementary and alternative medicine : eCAM·2019
Same author

Size-Dependent Relaxation Processes of Photoexcited [ n]Cycloparaphenylenes ( n = 5-12): Significant Contribution of Internal Conversion in Smaller Rings.

The journal of physical chemistry. A·2019
Same author

NfiR, a New Regulatory Noncoding RNA (ncRNA), Is Required in Concert with the NfiS ncRNA for Optimal Expression of Nitrogenase Genes in Pseudomonas stutzeri A1501.

Applied and environmental microbiology·2019
Same author

A novel homolateral and dicationic AIEgen for the sensitive detection of casein.

The Analyst·2019
Same author

Terahertz Oscilloscope for Recording Time Information of Ultrashort Electron Beams.

Physical review letters·2019
Same author

Flexible Transparent Organic Artificial Synapse Based on the Tungsten/Egg Albumen/Indium Tin Oxide/Polyethylene Terephthalate Memristor.

ACS applied materials & interfaces·2019

Related Experiment Video

Updated: Dec 27, 2025

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

7.2K

Visualization of materials using the confocal laser scanning microscopy technique.

Xu Teng1, Feng Li, Chao Lu

  • 1Beijing Advanced Innovation Center for Soft Matter Science and Engineering (BAICAS), State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China. luchao@mail.buct.edu.cn.

Chemical Society Reviews
|March 6, 2020
PubMed
Summary

Confocal laser scanning microscopy (CLSM) offers advanced, non-invasive material characterization. This review details CLSM

More Related Videos

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
10:53

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

10.2K
Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
07:04

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Published on: November 9, 2014

13.9K

Related Experiment Videos

Last Updated: Dec 27, 2025

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells

Published on: August 11, 2020

7.2K
Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
10:53

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

10.2K
Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
07:04

Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites

Published on: November 9, 2014

13.9K

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Engineering

Background:

  • Advanced characterization is crucial for materials science development.
  • Imaging techniques are vital for materials research.
  • Confocal laser scanning microscopy (CLSM) offers advantages over conventional methods.

Purpose of the Study:

  • To review recent applications of CLSM in materials science.
  • To guide researchers in selecting appropriate CLSM techniques for material characterization.
  • To inspire new strategies for advanced material preparation.

Main Methods:

  • Summarizing recent CLSM applications in materials science.
  • Discussing CLSM approaches for visualizing surface, internal, and spatial structures.
  • Detailing CLSM for observing reaction processes in materials.

Main Results:

  • CLSM provides non-invasive, high-contrast imaging with simple sample preparation.
  • It enables rapid acquisition of longitudinal and cross-sectional images.
  • CLSM offers direct, model-independent insights into material properties.

Conclusions:

  • CLSM is a powerful tool for materials characterization.
  • Future developments in CLSM will further advance materials science.
  • This review serves as a guide for researchers in materials science, chemistry, and engineering.