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Updated: Dec 27, 2025
A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
Published on: March 8, 2022
Long non-coding RNA FENDRR inhibits migration and invasion of cutaneous malignant melanoma cells
Xu-E Chen1, Pu Chen2, Shanshan Chen1
1Department of Dermatology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, China.
Abstract:
The present study aimed to investigate the effects of lncRNA FENDRR on the migration and invasion of malignant melanoma (MM) cells. The expression levels of FENDRR in MM tissues and MM cell lines were detected using qRT-PCR, followed by construction of FENDRR-knocked down and overexpressed stable cells. Then the effects of FENDRR on cell proliferation, migration and invasion were detected using MTT assay and Transwell assay. The protein expression levels of matrix metallopeptidase 2 (MMP2), MMP9, and related factors in JNK/c-Jun pathway were detected using Western blot. FENDRR was down-regulated in MM tissues and cell lines. Besides, its expression levels in different MM cells were diverse. Knockdown of FENDRR facilitated MM cells proliferation, migration and invasion in A375 cells, while overexpressing FENDRR had reverse results. In addition, MMPs and JNK/c-Jun pathway involved in the FENDRR-mediated regulation of MM cell proliferation, migration and invasion. Our results demonstrated that FENDRR mediated the metastasis phenotype of MM cells by inhibiting the expressions of MMP2 and MMP9 and antagonizing the JNK/c-Jun pathway.
Insights
Long non-coding RNA FENDRR inhibits malignant melanoma (MM) cell metastasis. Down-regulation of FENDRR promotes MM cell migration and invasion by activating MMP2, MMP9, and the JNK/c-Jun pathway.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Malignant melanoma (MM) is an aggressive skin cancer with high metastasis rates.
- The role of long non-coding RNAs (lncRNAs) in MM progression is increasingly recognized.
- FENDRR, a lncRNA, has been implicated in various cancers, but its function in MM requires further elucidation.
Purpose of the Study:
- To investigate the functional role of lncRNA FENDRR in the migration and invasion of malignant melanoma cells.
- To explore the underlying molecular mechanisms, including the involvement of matrix metalloproteinases (MMPs) and the JNK/c-Jun pathway.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) to assess FENDRR expression in MM tissues and cell lines.
- Cell proliferation, migration, and invasion assays (MTT and Transwell) in FENDRR-knocked down and overexpressed MM cells.
- Western blot analysis to detect protein expression of MMP2, MMP9, and JNK/c-Jun pathway components.
Main Results:
- FENDRR expression was significantly down-regulated in MM tissues and cell lines compared to normal controls.
- Knockdown of FENDRR enhanced MM cell proliferation, migration, and invasion.
- Overexpression of FENDRR suppressed these malignant phenotypes.
- FENDRR inhibited the expression of MMP2 and MMP9 and antagonized the JNK/c-Jun signaling pathway.
Conclusions:
- FENDRR acts as a tumor suppressor in malignant melanoma.
- FENDRR inhibits MM cell metastasis by down-regulating MMP2 and MMP9 expression and suppressing the JNK/c-Jun pathway.
- Targeting FENDRR may represent a potential therapeutic strategy for MM treatment.
