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Updated: Dec 27, 2025

Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

Published on: January 21, 2021

Ultrasound-Guided Cytosolic Protein Delivery via Transient Fluorous Masks.

Janna N Sloand¹, Theodore T Nguyen², Scott A Zinck³

¹Department of Biomedical Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.

|March 6, 2020

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Researchers developed ultrasound-sensitive nanoemulsions for precise protein delivery into cells. This technology enables image-guided cytosolic delivery of therapeutic proteins like antibodies, advancing precision medicine.

Area of Science:

Biotechnology
Nanomedicine
Biochemistry

Background:

Efficient protein delivery into cells is crucial for precision medicine.
Current methods face challenges in spatiotemporal control and cellular entry.
Targeted protein delivery can unlock new therapeutic and diagnostic applications.

Purpose of the Study:

To develop ultrasound-sensitive nanoemulsions for targeted protein delivery.
To enable on-demand cytosolic delivery of proteins, including antibodies, using diagnostic ultrasound.
To demonstrate real-time imaging-guided delivery and activation of protein complexes.

Main Methods:

Discovery of fluorous tags (FTags) for transient protein masking.
Formulation of ultrasound-sensitive fluoro-protein nanoemulsions in perfluorocarbons.

Keywords:

nanodroplets perfluorocarbon protein delivery theranostics ultrasound

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Utilizing clinically relevant diagnostic ultrasound for tracking, guidance, and activation.

Biochemical analysis of FTag masking and shedding.

In vitro and in vivo studies for cytosolic antibody delivery.

Main Results:

Fluoro-protein nanoemulsions allow acoustic tracking, guidance, and activation.
FTags enable efficient protein dispersion and are shed without compromising protein function.
Ultrasound sensitivity of nanoemulsions facilitates controlled cytosolic delivery of antibodies.
Real-time imaging monitors and activates FTag-protein complexes for precise delivery.

Conclusions:

Ultrasound-sensitive fluoro-protein nanoemulsions represent a novel platform for image-guided protein delivery.
This technology overcomes key barriers in spatiotemporal protein guidance and cellular delivery.
The findings hold potential for advancing protein-based biosensing and therapeutic modalities in precision medicine.