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Long non-coding RNA FEZF1-AS1 induced progression of ovarian cancer via regulating miR-130a-5p/SOX4 axis

Ziqian Sun1, Shouyang Gao1, Lili Xuan1

  • 1Department of Gynaecology and Obstetrics, China-Japan Union Hospital of Jilin University, Changchun, China.

Journal of Cellular and Molecular Medicine
|March 6, 2020
PubMed

Insights

Long non-coding RNA FEZF1-AS1 promotes ovarian cancer progression by enhancing cell proliferation and metastasis. This study reveals FEZF1-AS1 targets miR-130a-5p, impacting SOX4 expression in ovarian cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development.
  • The specific function of FEZF1 antisense RNA 1 (FEZF1-AS1) in ovarian cancer (OC) remains largely unknown.
  • Understanding lncRNA mechanisms is crucial for identifying novel therapeutic targets in epithelial ovarian cancer (EOC).

Purpose of the Study:

  • To elucidate the biological function and underlying molecular mechanisms of FEZF1-AS1 in ovarian cancer progression.
  • To investigate the expression patterns of FEZF1-AS1 in OC cell lines and patient tissues.
  • To explore the regulatory network involving FEZF1-AS1, microRNAs, and downstream target genes in OC.

Main Methods:

  • Quantitative analysis of FEZF1-AS1 expression in OC cell lines and human tissues.
  • In vitro functional assays (proliferation, invasion, migration, apoptosis, wound healing, colony formation) following FEZF1-AS1 knockdown.
  • Luciferase reporter assays to confirm direct interaction between FEZF1-AS1 and miR-130a-5p.
  • Bioinformatics analysis to predict and validate gene interactions.

Main Results:

  • FEZF1-AS1 was found to be overexpressed in OC cell lines and tissues.
  • Silencing FEZF1-AS1 significantly inhibited OC cell proliferation, invasion, migration, and colony formation, while promoting apoptosis.
  • FEZF1-AS1 was identified as a direct sponge for miR-130a-5p, suppressing its activity.
  • Overexpression of FEZF1-AS1 reversed the inhibitory effects of miR-130a-5p on OC cell growth by upregulating SOX4 expression.

Conclusions:

  • FEZF1-AS1 acts as an oncogenic lncRNA in ovarian cancer.
  • FEZF1-AS1 promotes OC cell proliferation and metastasis through the miR-130a-5p/SOX4 axis.
  • Targeting FEZF1-AS1 may represent a potential therapeutic strategy for epithelial ovarian cancer.
Keywords:
FEZF1 antisense RNA 1 (FEZF1-AS1)epithelial ovarian cancerinvasionmiR-130a-5pmigrationproliferation

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