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Colorimetric acid phosphatase sensor based on MoO3 nanozyme.

Zhen Lin1, Xiaomin Zhang2, Shijun Liu2

  • 1Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, 350122, China; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, 350122, China; Fujian Key Laboratory of Drug Target Discovery and Structural and Functional Research, Fujian Medical University, Fuzhou, 350122, China; Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical University, Fuzhou, 350122, China.

Analytica Chimica Acta
|March 7, 2020
PubMed
Summary
This summary is machine-generated.

Molybdenum oxide nanoparticles (MoO3 NPs) exhibit oxidase-mimicking activity, enabling a novel colorimetric sensor for acid phosphatase (ACP). This nanozyme-based method offers sensitive ACP detection in serum and inhibitor screening.

Keywords:
Acid phosphataseColorimetric assayMoO(3) nanoparticlesOxidase mimic

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Area of Science:

  • Nanomaterials Science
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Nanozymes offer stable, cost-effective alternatives to natural enzymes for biomedical applications.
  • Molybdenum oxide nanoparticles (MoO3 NPs) possess intrinsic oxidase-mimicking catalytic activity.
  • This activity involves the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) via singlet oxygen (1O2).

Purpose of the Study:

  • To investigate the oxidase-mimicking activity of MoO3 NPs.
  • To develop a novel colorimetric method for acid phosphatase (ACP) detection using MoO3 NPs.
  • To explore the application of this method in biological samples and inhibitor screening.

Main Methods:

  • Characterization of MoO3 NPs' oxidase-mimicking activity.
  • Electron spin resonance and radical inhibition assays to elucidate the catalytic mechanism.
  • Development of a colorimetric assay combining MoO3 NP activity and ACP-mediated substrate hydrolysis.
  • Application in diluted human serum samples and ACP inhibitor screening.

Main Results:

  • MoO3 NPs effectively catalyze ABTS oxidation through 1O2 generation.
  • Ascorbic acid (AA), produced by ACP-catalyzed hydrolysis of ascorbic acid 2-phosphate (AAP), inhibits the colorimetric reaction.
  • A sensitive colorimetric method for ACP detection was established with a linear range of 0.09–7.3 U/L and a detection limit of 0.011 U/L.
  • The method demonstrated successful application in detecting ACP in human serum and screening ACP inhibitors.

Conclusions:

  • MoO3 NPs serve as effective oxidase mimics for biosensing.
  • A simple, sensitive colorimetric method for ACP detection has been developed.
  • This approach holds promise for various biosensing applications and inhibitor screening.