Related Experiment Video
Updated: Dec 27, 2025
Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells
Published on: August 17, 2022
Expression of CD80 and CD86 on B cells during coxsackievirus B3-induced acute myocarditis
Yanlan Huang1, Bin Wei1, Xingcui Gao1
1Department of Cardiology, First Affiliated Hospital of Guangxi Medical University, Guangxi, China.
Introduction:
The pathogenesis of viral myocarditis (VMC) is unclear, but many studies have shown that VMC is associated with an excessive immune response. CD80 and CD86 are important costimulatory molecules that play a critical role in autoimmunity. However, whether CD80+/CD86+ B cells participate in the pathogenesis of acute VMC is unknown.
Material And Methods:
Male C57BL/6 mice were infected by intraperitoneal injection with coxsackievirus B3 (CVB3) to establish a VMC model. Control mice were administered phosphate-buffered saline intraperitoneally. At one week and two weeks post injection, histopathological changes in heart tissue were assessed with haematoxylin and eosin staining. The frequency of splenic CD80+/CD86+ B cells was measured with flow cytometry.
Results:
The frequency of CD80+ B cells was significantly increased in VMC, while the frequency of CD86+ B cells was significantly decreased. Furthermore, the frequency of CD80+ B cells related to the severity of VMC.
Conclusions:
These data show that CD80+/CD86+B cells are involved in the pathogenesis of VMC, with CD80+B cells being more important than CD86+B cells.
Related Concept Videos
01:27Myocarditis II: Clinical Features and Diagnostic Tests
01:21Myocarditis I: Introduction
01:14Myocarditis III: Medical Management
01:25Endocarditis II: Clinical Features of Infective Endocarditis
01:25Endocarditis I: Introduction
01:24B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...