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Updated: Dec 27, 2025
Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes hPSC-CMs Using Multi-electrode Arrays MEAs
Published on: May 12, 2017
A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM)
Alisha House1, Erica Fatica1, Rohan Shah1
1Department of Chemistry, Cleveland State University, Cleveland, OH 44115, United States.
Abstract:
Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.
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