Related Experiment Video
Updated: Dec 27, 2025
Author Spotlight: Establishing a New Fluorescence-Based Protocol for In Vivo Mitochondrial Morphology Analysis in Parkinson's Disease
Published on: June 23, 2023
MicroRNA-216a inhibits neuronal apoptosis in a cellular Parkinson's disease model by targeting Bax
Xiaobo Yang1, Meng Zhang2, Meng Wei2
1Department of Psychology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, People's Republic of China.
Abstract:
The study found that microRNAs play an important role in Parkinson's disease (PD). However, the function of MicroRNA-216a (miR-216a) in PD is unclear. Therefore, this experiment aimed to investigate the pathogenesis of miR-216a in PD. Using the toxicity of MPP+ to polyhexamine neurons, apoptosis of SH-SY5Y neuroblastoma cells was induced at different time by MPP+ to construct a stable acute PD cell model. The effects of DNA breakage, mitochondrial membrane potential (A ^ m), caspase-3 activity and nucleosome enrichment on cell apoptosis were detected by flow cytometry, TUNEL. MPP+ increased the toxic effects of dopaminergic neurons in a PD model. The introduction of miR-216a inhibited MPP + -induced neuronal apoptosis. The main manifestations were the decreased levels of positive rate of Tunel cells, caspase 3 activity and nucleosome enrichment factor. Bax was a direct target of miR-216a. In addition, Bax overexpression reversed the effects of miR-216a on neural cells. Bax downstream factors were also involved in miR-216a regulation of MPP + -triggered neuronal apoptosis. miR-216a regulated the progression of PD by regulating Bax, and miR-216a may be a potential target for PD.
Insights
MicroRNA-216a (miR-216a) protects against Parkinson's disease (PD) by inhibiting neuronal apoptosis. It targets Bax, suggesting miR-216a is a potential therapeutic target for PD.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- MicroRNAs (miRNAs) are implicated in Parkinson's disease (PD) pathogenesis.
- The specific role of MicroRNA-216a (miR-216a) in PD remains largely uncharacterized.
Purpose of the Study:
- To elucidate the role and mechanism of miR-216a in the development of Parkinson's disease.
- To investigate miR-216a's influence on neuronal apoptosis induced by MPP+.
Main Methods:
- Established an acute Parkinson's disease cell model using MPP+-induced apoptosis in SH-SY5Y neuroblastoma cells.
- Assessed apoptosis markers including DNA breakage (TUNEL), mitochondrial membrane potential, caspase-3 activity, and nucleosome enrichment.
- Utilized flow cytometry and TUNEL assays to quantify cellular apoptosis.
Main Results:
- MPP+ treatment significantly increased dopaminergic neuron toxicity and apoptosis.
- Introduction of miR-216a attenuated MPP+-induced neuronal apoptosis, reducing TUNEL-positive cells, caspase-3 activity, and nucleosome enrichment.
- Bax was identified as a direct target of miR-216a, and its overexpression reversed the protective effects of miR-216a.
Conclusions:
- miR-216a plays a protective role in Parkinson's disease by inhibiting neuronal apoptosis through the regulation of Bax.
- miR-216a represents a potential therapeutic target for Parkinson's disease treatment.
More Related Videos
15:09The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson's Disease
Published on: October 3, 2012
06:51Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents
Published on: August 10, 2018
Related Concept Videos
01:31The Intrinsic Apoptotic Pathway
01:22MicroRNAs
01:22MicroRNAs