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Hybridization kinetics of DNA fragments in the presence of ligands intercalating into DNA-duplexes
Valeri B Arakelyan1, Aleksandr Y Mamasakhlisov2, Eduard M Kazaryan2
1Faculty of Physics, Department of Molecular Physics, Yerevan State University, Yerevan, Armenia.
Abstract:
In this work the hybridization kinetics of DNA fragments on underlayer has been studied, when besides single-stranded DNA molecules there are also ligands in the medium that are able to intercalate into DNA-duplexes. A system of differential equations was obtained that describes the correlative change of the number of DNA-duplexes on the underlayer and the change of the number of ligands intercalating into DNA-duplexes. It was shown that the rate of underlayer filling by DNA-duplexes increases along with the enhancement of both equilibrium constant of formation reaction of DNA-duplexes and the concentration of DNA targets in the solution. It was also shown that the intercalation kinetics of ligands into DNA-duplexes relevantly depends on relation of dissociation rate constant of DNA-duplex to dissociation rate of the ligand complex with DNA-duplex.Communicated by Ramaswamy H. Sarma.
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