Related Experiment Video

Updated: Dec 27, 2025

In Vitro Analysis of E3 Ubiquitin Ligase Function
06:06

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

5.9K

The Role of Ubiquitin E3 Ligase in Atherosclerosis

Zhi-Xiang Zhou1, Zhong Ren1, Bin-Jie Yan1

  • 1Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerosis of Hunan Province, Hengyang Medical College, University of South China, Hengyang City, Hunan Province 421001, China.

Current Medicinal Chemistry
|March 7, 2020
PubMed

Insights

Ubiquitination, a protein modification, is crucial in atherosclerosis development. Targeting E3 ligases, key players in ubiquitination, offers a new strategy to prevent this vascular disease.

Area of Science:

  • Biochemistry
  • Cardiovascular Biology
  • Molecular Medicine

Background:

  • Atherosclerosis is a major global cause of death, driven by complex molecular mechanisms.
  • Key factors include abnormal lipid metabolism, inflammation, and cell dysfunction.
  • The precise molecular pathways, especially post-translational modifications, are not fully understood.

Purpose of the Study:

  • To review the role of ubiquitination in atherosclerosis pathogenesis.
  • To highlight the contributions of E3 ligases in this vascular disease.
  • To explore therapeutic potential of targeting E3 ligases.

Main Methods:

  • Literature review focusing on ubiquitination and E3 ligases in atherosclerosis.
  • Analysis of studies investigating molecular mechanisms of atherosclerosis.
  • Synthesis of current knowledge on ubiquitin-modifying enzymes.

Main Results:

  • Ubiquitination significantly impacts vascular inflammation and endothelial/smooth muscle cell function.
  • It plays a role in lipid metabolism and atherosclerotic plaque stability.
  • Various E3 ligases are implicated in the development of atherosclerosis.

Conclusions:

  • Ubiquitination is a critical regulator in atherosclerosis.
  • E3 ligases are key mediators in the disease's progression.
  • Targeting E3 ligases presents a promising therapeutic avenue for atherosclerosis prevention.

Keywords:
Atherosclerosisendothelial dysfunctionlipid metabolismubiquitin E3 ligasesvascular inflammationvascular smooth muscle cell function

More Related Videos

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates09:47

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

3.0K
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta10:27

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Published on: December 5, 2019

9.3K

Related Concept Videos

Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.6K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.7K
Atherosclerosis III: Management01:26

Atherosclerosis III: Management

Management of atherosclerosis involves an integrated strategy encompassing pharmacological treatment, surgical interventions, lifestyle changes, and nutrition therapy to address the multifactorial nature of the disease.Pharmacological TherapyA cornerstone of atherosclerosis management is the use of pharmacological agents. Statins, such as atorvastatin, are pivotal in inhibiting HMG-CoA reductase, an enzyme that catalyzes an initial step in cholesterol synthesis in the liver. This reduction in...
272
Atherosclerosis I: Introduction01:30

Atherosclerosis I: Introduction

Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...
684
The Proteasome01:13

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
1.5K
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
9.9K