Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complement System01:27

Complement System

8.9K
The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
8.9K
Antibody Actions01:26

Antibody Actions

2.2K
Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
2.2K
Humoral Immune Responses01:36

Humoral Immune Responses

83.0K
Overview
83.0K
Antimicrobial Proteins01:23

Antimicrobial Proteins

12.8K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
12.8K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

15.6K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
15.6K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

2.5K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Drawing a line in the sand: impact of analytical choices on anti-drug antibody cut-points and testing outcomes.

Bioanalysis·2026
Same author

Bispecific 10E8.4/iMab broadly neutralizing antibody in people with or without HIV-1: a partially randomized phase 1 trial.

Nature medicine·2026
Same author

RAD: A Read-structure Agnostic Demultiplexer for Single-Cell Long-Read Sequencing and Analysis.

bioRxiv : the preprint server for biology·2026
Same author

Characterization of Anti-Canine PD-1 Antibodies.

Cells·2026
Same author

Depletion and recovery of IgG following treatment with an anti-FcRn antibody and IdeS in pigtail macaques.

Clinical and experimental immunology·2026
Same author

Prefusion-specific glycoprotein B human antibodies protect against neonatal HSV-2 infection.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Dec 26, 2025

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
06:29

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

31.0K

Antibody-mediated complement activation in pathology and protection.

Benjamin S Goldberg1, Margaret E Ackerman1,2

  • 1Thayer School of Engineering, Dartmouth College, Hanover, NH, USA.

Immunology and Cell Biology
|March 7, 2020
PubMed
Summary

Antibody-dependent complement activity influences autoimmune diseases and cancer treatment. Understanding antibody interactions with complement component 1q is key to developing new therapies for challenging diseases.

Keywords:
Antibody-dependent complement activationantibody engineeringcancercomplementinfectious disease

More Related Videos

In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis
07:25

In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis

Published on: May 4, 2017

18.1K
High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

12.1K

Related Experiment Videos

Last Updated: Dec 26, 2025

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
06:29

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

31.0K
In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis
07:25

In Vitro Methods for Comparing Target Binding and CDC Induction Between Therapeutic Antibodies: Applications in Biosimilarity Analysis

Published on: May 4, 2017

18.1K
High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

12.1K

Area of Science:

  • Immunology
  • Molecular Biology
  • Structural Biology

Background:

  • Antibody-dependent complement activity plays a dual role in autoimmune morbidity and antitumor efficacy.
  • Both natural and engineered antibodies can mediate complement activity, impacting infectious diseases, autoimmunity, and cancer.
  • Recent structural studies reveal molecular details of antibody-mediated complement activation.

Purpose of the Study:

  • To explore the structural and functional factors governing antibody-mediated complement activation.
  • To understand the diverse roles of complement activation products in cellular interactions and disease.
  • To highlight therapeutic strategies for modulating complement activity in antibody-based treatments.

Main Methods:

  • Structural resolution of molecular complexes involved in complement activation.
  • Analysis of factors influencing antibody multimerization and complement component 1q recruitment.
  • Investigation of complement activation product interactions with various cell types.

Main Results:

  • Identified key factors (isotype, glycosylation, antigen context) determining complement activity.
  • Demonstrated diverse cellular interactions mediated by complement activation products.
  • Highlighted the dual role of complement in homeostasis, infection, and tumorigenesis.

Conclusions:

  • Understanding antibody-complement interactions is crucial for optimizing antibody-based therapies.
  • Therapeutic strategies include suppressing complement activation (e.g., IVIG) or antibody engineering.
  • Advances promise better treatments for autoimmune diseases, infections, and cancers by refining antibody drug mechanisms.