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Updated: Dec 26, 2025

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides

Published on: August 20, 2018

Enabling sublingual peptide immunization with molecular self-assemblies.

Sean H Kelly¹, Yaoying Wu¹, Ajay K Varadhan¹

¹Biomedical Engineering Department, Duke University, Durham, NC, 27708, United States.

|March 7, 2020

Summary

This summary is machine-generated.

Sublingual peptide vaccines are enhanced by assembling peptides into nanofibers with specific PEGylation. This nanomaterial design improves immunogenicity and immune responses, overcoming previous delivery challenges.

Keywords:

Mucosal Nanofiber Self-assembly Sublingual Supramolecular Vaccine

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Last Updated: Dec 26, 2025

Synthesis and Characterization of 1,2-Dithiolane Modified Self-Assembling Peptides

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Published on: August 20, 2018

Author Spotlight: Improving the Production of Self-Assembling Fibers and Peptide Hydrogels for Superior Biocompatibility

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Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

Area of Science:

Biomaterials Science
Vaccinology
Immunology

Background:

Short peptides exhibit low immunogenicity via sublingual delivery.
Nanomaterial delivery faces challenges in sublingual vaccine development due to mucosal transport barriers.

Purpose of the Study:

To enhance the immunogenicity of peptides for sublingual vaccine applications.
To investigate the role of nanomaterial assembly and surface properties in sublingual vaccine efficacy.

Main Methods:

Assembly of peptides into supramolecular polymer-peptide nanofibers with varying PEGylation.
Evaluation of nanofiber-mucin interactions and residence time.
Assessment of systemic and mucosal antibody and T-cell responses in mice.

Main Results:

Nanofiber assembly dramatically enhanced sublingual peptide immunogenicity, unlike PEGylation or adjuvants alone.
Optimized PEGylation (2000-3000 Da) reduced mucin interaction and increased residence time.
Nanofiber vaccines elicited strong, durable systemic and mucosal immune responses, including T-cell responses.
PASylation offered an alternative to PEGylation for achieving sublingual immunogenicity.

Conclusions:

Supramolecular assembly into nanofibers, particularly with optimized PEGylation, is critical for effective sublingual peptide vaccines.
Surface properties, not size, are key in modulating sublingual nanomaterial immunogenicity.
This approach has significant implications for designing novel synthetic sublingual vaccines.