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Measuring bioconcentration factors of sediment-associated fipronil in Lumbriculus variegatus using passive sampling
Shunhui Wang1, Wenjian Lao2, Huizhen Li3
1School of Environment and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou, 511443, China; School of Chemistry and Chemical Engineering, Oil & Gas Field Applied Chemistry Key Laboratory of Sichuan Province, Southwest Petroleum University, Chengdu, 610500, China.
Abstract:
Fipronil and its degradates have been detected ubiquitously in aquatic environment worldwide, yet little is known about its bioaccumulation potential. The goal of the present study was to measure bioconcentration factor (BCF) of sediment-associated fipronil in a benthic invertebrate, Lumbriculus variegatus using passive sampling techniques. Three passive samplers including polymethyl methacrylate (PMMA) film, poly(dimethylsiloxane) fiber and polyacrylate fiber were evaluated. PMMA film was identified as the preferred method and was applied to determine fipronil log KOC (3.77 ± 0.04). BCF of sediment-associated fipronil in L. variegatus was obtained through measuring freely dissolved concentration (Cfree). Because fipronil degraded in sediment, time weighted average (TWA) Cfree was estimated for calculating BCFTWA (1855 ± 293 mL/g lipid). Fipronil BCF was also measured in a water-only bioaccumulation test of L. variegatus under constant exposure condition. This BCF value (1892 ± 76 mL/g lipid) was comparable with the BCFTWA, validating effectiveness of the passive sampling method for the measurement of sediment Cfree. Fipronil was bioaccumulative in L. variegatus according to the USEPA's criteria. The combination of Cfree and TWA concentration measurements was demonstrated to properly determine BCF value for moderately hydrophobic and degradable chemicals in sediment.
