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Tissue Engineered Esophageal Patch by Mesenchymal Stromal Cells: Optimization of Electrospun Patch Engineering.

Silvia Pisani1, Stefania Croce2, Enrica Chiesa1

  • 1Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy.

International Journal of Molecular Sciences
|March 8, 2020
PubMed
Summary

A new static seeding protocol improves cellularization of biodegradable tissue engineering scaffolds. This simple, reproducible method enhances cell distribution and minimizes damage for better in vitro results.

Keywords:
electrospinningpatch engineeringporcine mesenchymal stem cellstemperature induced precipitation

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Developing functional tissue engineering scaffolds requires efficient cell seeding and uniform cellularization.
  • Biodegradable patches need robust protocols to avoid structural damage and contamination during culture.

Purpose of the Study:

  • To establish a simple, reproducible protocol for uniform cell seeding and optimal cellularization of biodegradable patches.
  • To minimize structural damage and contamination risks in long-term cell cultures of engineered scaffolds.

Main Methods:

  • Comparison of static seeding in free-floating versus fixed (CellCrown™ insert) conditions using porcine bone marrow MSCs (p-MSCs).
  • Fabrication of scaffold prototypes using temperature-induced precipitation and electrospinning, analyzing pore size, orientation, porosity, and distribution.
  • Evaluation of cell distribution uniformity, seeding efficiency, and cellularization timing.

Main Results:

  • Static seeding conditions significantly improved the cellularization of polymeric patches.
  • The developed protocol demonstrated suitability for achieving homogeneous and complete patch cellularization in vitro.
  • Scaffold prototypes exhibited stability under physiological simulating conditions.

Conclusions:

  • The proposed in vitro protocol is effective for homogeneous and complete cellularization of biodegradable patches.
  • The static seeding method is simple, repeatable, and reproducible, offering a reliable approach for tissue engineering applications.