Tn402: a new transposable element determining trimethoprim resistance that inserts in bacteriophage lambda.

Area of Science:

• Microbiology
• Molecular Biology
• Genetics

Background:

• The IncP plasmid R751 carries a trimethoprim resistance determinant.
• Transposable elements play a crucial role in the dissemination of antibiotic resistance.

Purpose of the Study:

• To investigate the transposable nature of the trimethoprim resistance determinant from plasmid R751.
• To characterize this novel transposable element.

Main Methods:

• Plasmid R751 DNA was used in transposition experiments.
• Bacteriophage lambda was employed as a recipient system.
• Molecular analysis was performed to identify and characterize the transposed element.

Main Results:

• The trimethoprim resistance determinant of IncP plasmid R751 was successfully transposed to bacteriophage lambda.
• The transposing unit was identified and designated as Tn402.
• This finding demonstrates the mobility of antibiotic resistance genes on plasmids.

Conclusions:

• The trimethoprim resistance determinant of R751 is a transposable element, designated Tn402.
• Tn402 can transpose to bacteriophage lambda, indicating its potential for wider dissemination.
• This discovery contributes to understanding the mechanisms of antibiotic resistance gene spread.