Related Experiment Video
Updated: Dec 26, 2025
09:39
Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
Published on: December 20, 2014
15.7K
Endocytic membrane trafficking in the control of centrosome function
Naava Naslavsky1, Steve Caplan1
1The Department of Biochemistry and Molecular Biology and Fred and Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, 68198-5870, United States.
Current Opinion in Cell Biology
|March 8, 2020
Summary
Endocytic membrane trafficking (EMT) proteins regulate more than just vesicles, influencing non-membrane organelles like the centrosome. This review explores EMT
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Endocytic trafficking was traditionally confined to membrane-bound organelles.
- Emerging evidence reveals endocytic regulatory proteins impact diverse cellular pathways beyond the endocytic route.
- These proteins also influence trafficking to and from non-membrane organelles, including the centrosome.
Purpose of the Study:
- To review the direct influence of endocytic membrane trafficking (EMT) on centrosome biology.
- To highlight the expanding roles of endocytic regulators in centrosome biogenesis, maintenance, cell cycle control, and duplication.
Main Methods:
- Literature review of recent studies on endocytic trafficking and centrosome regulation.
- Analysis of implicated endocytic regulatory proteins, such as Rab proteins (e.g., Rab11) and leucine-rich repeat kinase 2.
Main Results:
- Endocytic regulatory proteins are increasingly implicated in centrosome regulation.
- EMT directly impacts centrosome biogenesis, cell cycle control, and duplication.
- Specific proteins like Rab11 and LRRK2 are involved in linking EMT to centrosome function.
Conclusions:
- Endocytic membrane trafficking plays a direct and significant role in centrosome regulation.
- Further research into the interplay between EMT and centrosomes is warranted to understand fundamental cellular processes.
Related Concept Videos
01:13Centrioles and Centrosomes
4.9K
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
Near the end of the prophase, also called late prophase or...
4.9K
02:25Centrosome Duplication
4.7K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
4.7K
01:32Cytoskeletal Coordination in Cell Migration
5.3K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.3K
01:44Introduction to Membrane Traffic
9.0K
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
9.0K
01:32Microtubule Associated Motor Proteins
9.8K
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
9.8K
01:24Microtubules in Cell Motility
4.4K
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
4.4K