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Aptamer-based ellipsometric sensor for ultrasensitive determination of aminoglycoside group antibiotics from dairy
1Bioengineering Department, Bilecik Seyh Edebali University, Bilecik, Turkey.
Background:
Residual antibiotics taken along with food consumed through the food chain are the main cause of the super-bacteria and may damage organs such as liver and kidney. Therefore, monitoring residual antibiotic levels of products in the food chain is both important and a requirement. Maximum residual limits for kanamycin and neomycin are 150 ng mL-1 and 500 ng mL-1 respectively, which are challenging for most sensor platforms. In this paper, a novel method is presented for the determination of antibiotics residues in animal-derived foods.
Results:
Aptamer-based kanamycin and neomycin biosensors based on the spectroscopic ellipsometer and the surface plasmon resonance-enhanced total internal reflection ellipsometer methods as transducing element were developed. Detection limits of both sensor platforms were in the 0.1-1 nmol L-1 ranges, and the detection range was between the detection limit and 1000 nmol L-1 .
Conclusion:
Both ellipsometry-based aptasensors can be used as an alternative to the existing enzyme-linked immunosorbent assay-based method in terms of assay time (10 min), detection limit (0.22 ng mL-1 for neomycin and 0.048 ng mL-1 for kanamycin), and detection range. © 2020 Society of Chemical Industry.
Insights
New aptasensors detect residual kanamycin and neomycin antibiotics in food. These biosensors offer a faster, more sensitive alternative to current methods for monitoring food safety and preventing super-bacteria development.
Area of Science:
- Analytical Chemistry
- Biosensor Technology
- Food Safety
Background:
- Antibiotic residues in food pose risks, contributing to super-bacteria and potential organ damage.
- Monitoring these residues is crucial for public health and regulatory compliance.
- Existing methods struggle to meet the stringent detection limits for antibiotics like kanamycin and neomycin.
Purpose of the Study:
- To develop novel, highly sensitive biosensors for detecting kanamycin and neomycin residues.
- To provide a rapid and reliable method for antibiotic residue analysis in animal-derived foods.
Main Methods:
- Development of aptamer-based biosensors utilizing spectroscopic ellipsometry and surface plasmon resonance-enhanced total internal reflection ellipsometry.
- Testing sensor performance for detection limits and dynamic ranges.
Main Results:
- Achieved detection limits in the 0.1-1 nmol L⁻¹ range for both kanamycin and neomycin.
- Demonstrated a wide detection range from the limit of detection up to 1000 nmol L⁻¹.
Conclusions:
- Ellipsometry-based aptasensors provide a viable alternative to ELISA for antibiotic residue detection.
- The developed sensors offer significant improvements in assay time (10 min) and sensitivity (0.048 ng mL⁻¹ for kanamycin, 0.22 ng mL⁻¹ for neomycin).
- These advanced biosensors enhance food safety monitoring capabilities.
