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Updated: Dec 26, 2025

Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells
Published on: June 14, 2022
[Modelling the bronchial epithelium in chronic obstructive pulmonary disease using human induced pluripotential stem
M Fieldès1, E Ahmed2, C Bourguignon1
1IRMB (Institut de Médecine Régénératrice et de Biothérapies de Montpellier), UMR 1183, CHU Montpellier, Montpellier, France.
Abstract:
Chronic obstructive pulmonary disease (COPD) is a chronic lung disease leading to irreversible destruction of the terminal bronchioles. Although the precise patho-physiological mechanisms remain to be elucidated, the bronchial epithelium seems to play a pivotal role in the disease. Recent studies have highlighted a great heterogeneity among COPD patients, with various disease courses including, in about half the cases, an origin in childhood. Modelling of COPD is a major goal but currently available models are imperfect. Our work aims to create a new in vitro cellular model to study the pathology of the disease. The differentiation of human induced pluripotential stem cells (hiPSCs) in bronchial epithelium is a step towards a better understanding of the developmental origin and the identification of new therapeutic targets.
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