Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development of High-Affinity CHD1 Chromodomain Inhibitors.

Journal of medicinal chemistry·2026

Same author

A versatile acoustic droplet ejection mass spectrometry platform enables drug discovery for the solute carrier (SLC) target family.

SLAS discovery : advancing life sciences R & D·2026

Same author

AI-Based Prediction of PROTAC- and Molecular Glue-Mediated Ternary Complexes: A Comparative Evaluation of AlphaFold 3 and Boltz-2.

Archiv der Pharmazie·2026

Same author

From Pharmacophore to Warhead: NAD<sup>+</sup>-Targeting Triazoles as Mechanism-Based Sirtuin Inhibitors.

Angewandte Chemie (International ed. in English)·2025

Same author

Benzodeazaoxaflavin Sirtuin Inhibitors Inhibit <i>Schistosoma mansoni</i> Sirt2 and Cause Phenotypic Changes and Lethality in Schistosomula and Adult Worm Stages.

ACS infectious diseases·2025

Same author

Novel selective indole based histone deacetylase 10 inhibitors as anticancer therapeutics.

Scientific reports·2025

Search research articles

Related Experiment Video

Updated: Dec 26, 2025

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

Proteolysis targeting chimeras (PROTACs) for epigenetics research.

Anja Vogelmann¹, Dina Robaa², Wolfgang Sippl²

¹Institute of Pharmaceutical Sciences, University of Freiburg, Albertstraße 25, Freiburg, 79104, Germany.

Current Opinion in Chemical Biology

|March 9, 2020

Summary

This summary is machine-generated.

Proteolysis targeting chimeras (PROTACs) offer a novel method for targeted protein degradation. This review highlights recent advancements in PROTACs, particularly for epigenetic targets in drug discovery.

Keywords:

Drug discovery Epigenetics PROTACs Protein degradation

More Related Videos

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

Related Experiment Videos

Last Updated: Dec 26, 2025

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

Published on: January 12, 2024

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines

Published on: November 9, 2020

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

Area of Science:

Biochemistry
Molecular Biology
Drug Discovery

Background:

Proteolysis targeting chimeras (PROTACs) are heterobifunctional molecules that induce selective protein degradation via the ubiquitin-proteasome system.
This chemical biology strategy has broad applications, targeting various proteins like receptors, kinases, and epigenetic factors.

Purpose of the Study:

To review and summarize recent developments in PROTAC discovery.
To focus specifically on PROTACs targeting epigenetic proteins.

Main Methods:

Literature review of recent scientific publications on PROTACs.
Analysis of PROTAC design, synthesis, and characterization in the context of epigenetics.

Main Results:

PROTACs have been successfully applied to a diverse range of targets, including epigenetic proteins.
Significant progress in PROTAC synthesis and characterization has led to improved molecular design and mechanistic understanding.

Conclusions:

PROTACs represent a powerful tool for chemical biology and drug discovery.
The field of epigenetic PROTACs is rapidly advancing, offering new therapeutic possibilities.