Related Experiment Videos
Cholinergic muscarinic receptors in rat cochlea
Y J van Megen1, A B Klaassen, J F Rodrigues de Miranda
1Department of Otorhinolaryngology, University of Nijmegen, The Netherlands.
Brain Research
|November 22, 1988
Abstract:
Specific 3H-1-quinuclidinylbenzilate (3H-1-QNB) binding to rat cochlea homogenates occurs to a homogeneous class of binding sites with Kd = 0.13 +/- 0.01 nM and Bmax = 0.57 +/- 0.07 fmol per cochlea. Binding is stereoselectively inhibited by benzetimide enantiomers. Dexetimide was more effective than levetimide in displacing 3H-1-QNB from its binding sites (Ki = 4 x 10(-10) M and 6.5 x 10(-6) M, respectively). Pirenzepine inhibits 3H-1-QNB binding with low affinity (Ki = 2 x 10(-6) M), classifying the binding sites as muscarinic M2 receptors.