Determining Coreceptor Expression and Function in Murine ILC2 Through Flow Cytometry Characterization and Coculture...

Group 2 innate lymphoid cells are important innate effectors and regulators of adaptive immunity in response to parasitic infections and allergic inflammation. Their low frequency in vivo during steady state condition may complicate research on the cells. During type 2 biased immune responses they are activated, increase in frequency and release cytokines as well as regulate T cell functions through direct interactions including MHC class II-T cell receptor interactions. Importantly, coreceptors significantly influence the ILC2-T cell cross talk and shape the adaptive immune response.Here, we provide an experimental framework to study the function of coreceptors expressed on tissue ILC2. In brief, we describe flow cytometric analysis of the coreceptor of interest, the isolation and culture of mouse pulmonary ILC2 and splenic T cells, as well as approaches to manipulate their coculture. Finally, downstream readout options are outlined.