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Related Experiment Video

Updated: Dec 26, 2025

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Determining Coreceptor Expression and Function in Murine ILC2 Through Flow Cytometry Characterization and Coculture

Christian Schwartz1,2, Padraic G Fallon3,4,5

  • 1Mikrobiologisches Institut-Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen and Friedrich-Alexander Universität (FAU) Erlangen-Nürnberg, Erlangen, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|March 10, 2020
PubMed
Summary

This study introduces a framework for investigating how coreceptors on group 2 innate lymphoid cells (ILC2s) influence immune responses. Understanding this interaction is key for studying parasitic infections and allergic inflammation.

Keywords:
CocultureCytokinesILC2Innate immunityMHC2RegulationStimulationT helper cellsTranscription factorsType 2 immune response

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Group 2 innate lymphoid cells (ILC2s) are crucial immune cells involved in parasitic infections and allergic inflammation.
  • Their low frequency in normal conditions poses research challenges.
  • ILC2s activate during type 2 immune responses, releasing cytokines and modulating T cell function via interactions like MHC class II-TCR.

Purpose of the Study:

  • To establish an experimental framework for studying the function of coreceptors on tissue-resident ILC2s.
  • To facilitate research on ILC2-T cell crosstalk and its impact on adaptive immunity.

Main Methods:

  • Flow cytometry for analyzing coreceptor expression.
  • Isolation and culture of mouse pulmonary ILC2s and splenic T cells.
  • Co-culture systems to study ILC2-T cell interactions and downstream readouts.

Main Results:

  • The study outlines a practical experimental approach for ILC2 coreceptor research.
  • The framework allows for manipulation of ILC2-T cell cocultures.
  • Provides options for analyzing the functional consequences of these interactions.

Conclusions:

  • This framework enables detailed investigation of coreceptor function on ILC2s.
  • It supports research into how ILC2-T cell interactions shape adaptive immunity.
  • Crucial for understanding immune responses in allergy and parasitic infections.