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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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[Advances in Clostridioes difficile genome editing].

Wei Hong1,2, Wen Wan3, Guzhen Cui4

  • 1Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang 550004, Guizhou, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|March 10, 2020
PubMed
Summary
This summary is machine-generated.

Genome editing in Clostridioides difficile (C. difficile) is crucial for understanding its pathogenicity. This review covers C. difficile genome-editing technologies, their progress, and future directions.

Keywords:
Clostridioes difficileClostridioes difficile infection (CDI)gene function researchgenome editingpathogenic mechanism

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Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • Clostridioides difficile is a leading cause of hospital-associated diarrhea.
  • Increased prevalence and mortality linked to virulent strains like ribotype 027.
  • Urgent need for genome-editing tools to study C. difficile physiology and pathogenesis.

Purpose of the Study:

  • To review the history, recent advancements, and future prospects of genome-editing technologies for C. difficile.
  • To highlight the importance of genome editing in dissecting C. difficile's mechanisms.
  • To discuss the role of specific tools like ClosTron in studying toxin-pathogenicity relationships.

Main Methods:

  • Review of existing literature on C. difficile genome editing.
  • Analysis of ClosTron technology and its application.
  • Discussion of historical development and current progress in the field.

Main Results:

  • Genome-editing technologies are essential for understanding C. difficile.
  • ClosTron technology is a key tool for studying C. difficile toxins and pathogenicity.
  • Significant progress has been made in developing stable and efficient genome-editing methods.

Conclusions:

  • Genome editing is vital for advancing C. difficile research.
  • Future prospects involve refining existing tools and developing novel genome-editing strategies.
  • Enhanced understanding of C. difficile pathogenesis will aid in developing new treatments.