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Published on: September 12, 2015
[Paeoniflorin improves the permeability of cardiac microvascular endothelial cells by regulating Src/vascular
Xiufang Hong1,2, Li Li1, Dongyang Guo1
1Department of Critical Care Medicine, Zhejiang Hospital, Hangzhou 310013, Zhejiang, China.
Objective:
To investigate the effect and mechanism of paeoniflorin on the permeability of cardiac microvascular endothelial cells (CMECs) in sepsis.
Methods:
Primary rat CMECs were isolated and cultured in vitro, and the cells in the logarithmic growth phase were used for experiments. Tetramethylazozolium colorimetry (MTT) was used to screen the safe and effective concentrations of paeoniflorin at 10, 20, and 40 μmol/L. The cells were divided into blank control group, lipopolysaccharide (LPS) group and low, medium and high concentration paeoniflorin pretreatment group. The cells in the blank control group were cultured in complete medium; the cells in the LPS group were challenged with LPS (1 mg/L) in complete medium; and the cells in the paeoniflorin pretreatment groups were pretreated with 10, 20, and 40 μmol/L paeoniflorin at 4 hours before LPS stimulation. The cells in each group were further cultured for 24 hours after LPS stimulation. The horseradish peroxidase (HRP) method was used to detect the permeability of rat CMECs. The enzyme-linked immunosorbent assay (ELISA) was used to detect the CXC chemokine ligand (CXCL1, CXCL2) levels in the cell supernatant. The real-time fluorescence quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was used to detect the mRNA expressions of CXCL1 and CXCL2 in the cells. Western Blot was used to detect phosphorylated Src (p-Src), vascular endothelial-cadherin (VE-cadherin) and phosphorylated mitogen activated protein kinase (p-MAPK).
Results:
Compared with the blank control group, the permeability of rat CMECs in the LPS group was significantly increased. The cell permeability was improved to some extent after paeoniflorin pretreatment at different concentrations, and the improvement was most obvious in the 40 μmol/L paeoniflorin group, with statistically significant difference as compared with the LPS group (A value: 1.61±0.07 vs. 2.13±0.06, P < 0.01). ELISA results showed that there were moderate amounts of CXCL1 and CXCL2 in the cell supernatant of rat CMECs in the blank control group. However, the secretion of CXCL1 and CXCL2 in the cell supernatant was increased significantly under the induction of LPS. After pretreatment with paeoniflorin at different concentrations, the secretion of CXCL1 and CXCL2 in the cell supernatant was significantly reduced. The most obvious inhibitory effect on CXCL1 was 40 μmol/L paeoniflorin, and the most obvious inhibition on CXCL2 was 20 μmol/L paeoniflorin, the differences were statistically significant as compared with the LPS group [CXCL1 (ng/L): 337.51±68.04 vs. 829.86±65.06, CXCL2 (ng/L): 4.48±0.11 vs. 9.41±0.70, both P < 0.01]. RT-qPCR results showed that the mRNA expressions of CXCL1 and CXCL2 in the rat CMECs were consistent with the ELISA results. LPS could increase mRNA expressions of CXCL1 and CXCL2 in the rat CMECs, and pretreatment with different concentrations of paeoniflorin could significantly reduce the mRNA expressions of CXCL1 and CXCL2. The 40 μmol/L paeoniflorin had the best inhibitory effect on CXCL1 mRNA expression, and the 20 μmol/L paeoniflorin had the best inhibitory effect on CXCL2 mRNA expression, the differences were statistically significant as compared with the LPS group [CXCL1 mRNA (2-ΔΔCt): 0.543±0.004 vs. 0.812±0.089, CXCL2 mRNA (2-ΔΔCt): 10.52±0.71 vs. 17.68±1.09, both P < 0.01]. Western Blot results showed that moderate amounts of p-Src, VE-cadherin and p-MAPK proteins were expressed in the rat CMECs in the blank control group. After LPS stimulation, the expressions of p-Src and p-MAPK proteins were increased significantly, while the expression of VE-cadherin protein was decreased significantly. After pretreatment with different concentrations of paeoniflorin, the expressions of p-Src and p-MAPK proteins in the cells were decreased to varying degrees, while the expression of VE-cadherin protein was increased, and 40 μmol/L paeoniflorin had the most obvious effect, the differences were statistically significant as compared with the LPS group [p-Src protein (p-Src/GAPDH): 1.02±0.09 vs. 1.29±0.05, p-MAPK proteins (p-MAPK/GAPDH): 0.24±0.02 vs. 0.62±0.02, VE-cadherin protein (VE-cadherin/GAPDH): 0.64±0.03 vs. 0.31±0.02, all P < 0.01].
Conclusions:
Paeoniflorin can regulate the Src/VE-cadherin pathway in CMECs, inhibit the expression and secretion of inflammation-related proteins and chemokines, and improve the cell permeability of CMECs induced by LPS.
Insights
Paeoniflorin effectively reduces cardiac microvascular endothelial cell (CMEC) permeability in sepsis by inhibiting inflammation-related proteins and chemokines. This study highlights paeoniflorin
Area of Science:
- Cardiovascular Biology
- Sepsis Pathophysiology
- Endothelial Cell Biology
Background:
- Sepsis-induced cardiac microvascular endothelial cell (CMEC) dysfunction increases vascular permeability.
- Understanding the molecular mechanisms underlying CMEC dysfunction is crucial for developing therapeutic strategies.
- Paeoniflorin, a natural compound, has shown potential anti-inflammatory effects.
Purpose of the Study:
- To investigate the therapeutic effect of paeoniflorin on CMEC permeability in a sepsis model.
- To elucidate the underlying molecular mechanisms involving inflammation-related proteins and signaling pathways.
Main Methods:
- Primary rat CMECs were isolated and treated with lipopolysaccharide (LPS) to induce sepsis-like conditions.
- Cells were pretreated with varying concentrations of paeoniflorin (10, 20, 40 μmol/L).
- Cell permeability, inflammatory chemokine (CXCL1, CXCL2) expression (ELISA, RT-qPCR), and key protein levels (p-Src, VE-cadherin, p-MAPK via Western Blot) were assessed.
Main Results:
- LPS significantly increased CMEC permeability, chemokine expression, and activated the Src/MAPK pathway (increased p-Src, p-MAPK; decreased VE-cadherin).
- Paeoniflorin pretreatment dose-dependently reduced CMEC permeability and suppressed CXCL1 and CXCL2 expression and secretion.
- Paeoniflorin effectively modulated the Src/VE-cadherin pathway, decreasing p-Src and p-MAPK, and increasing VE-cadherin expression.
Conclusions:
- Paeoniflorin demonstrates a protective effect against LPS-induced CMEC injury in sepsis.
- The mechanism involves the regulation of the Src/VE-cadherin signaling pathway.
- Paeoniflorin inhibits the expression and secretion of inflammatory mediators, thereby improving CMEC barrier function.
