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MICROSCALE SERUM EXTRACTION METHOD FOR THE SIMULTANEOUS ANALYSIS OF CORTICOSTERONE AND LIPIDS
Alana L Rister1, Katie L Bidne2, Jennifer R Wood2
1Department of Chemistry, University of Nebraska - Lincoln, Lincoln, NE, 68588-0304, USA.
Analytical Methods : Advancing Methods and Applications
|March 10, 2020
Summary
This study presents a new method for simultaneously measuring corticosterone and lipids in small serum samples. The technique uses a modified Folch extraction followed by liquid chromatography-mass spectrometry (LC-MS) for accurate quantification.
Area of Science:
- Biochemistry
- Analytical Chemistry
Background:
- Corticosterone regulates metabolism and stress response.
- Current measurement methods like RIA and ELISA have limitations including radioactivity, cost, and single-analyte measurement.
Purpose of the Study:
- To develop a novel, efficient method for simultaneous quantification of corticosterone and lipids from small serum volumes.
- To overcome the drawbacks of existing corticosterone measurement techniques.
Main Methods:
- A modified Folch extraction technique was employed for simultaneous isolation of corticosterone and lipids from serum.
- Liquid chromatography-mass spectrometry (LC-MS) was used for the analysis of the extracted samples.
Main Results:
- The method successfully quantified corticosterone using only 5 µL of serum.
- Achieved coefficients of variation of 3% or less and a detection limit of 0.12 µM for corticosterone.
- Enabled simultaneous isolation and measurement of both corticosterone and lipids.
Conclusions:
- The developed method offers a beneficial approach for measuring circulating corticosterone and lipids, particularly in studies with limited sample volumes.
- This technique addresses the limitations of traditional methods, offering improved efficiency and cost-effectiveness.
