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Identification of Coronavirus Isolated from a Patient in Korea with COVID-19

Jeong-Min Kim1, Yoon-Seok Chung1, Hye Jun Jo1

  • 1Division of Viral Diseases, Center for Laboratory Control of Infectious Diseases, Korea Centers for Disease Control and Prevention, Cheongju, Korea.

Osong Public Health and Research Perspectives
|March 10, 2020
PubMed

Insights

Researchers successfully isolated SARS-CoV-2, the virus causing COVID-19, from patients in South Korea. This isolation aids in understanding and combating the novel coronavirus pandemic.

Area of Science:

  • Virology
  • Infectious Diseases
  • Public Health

Background:

  • The emergence of coronavirus disease 2019 (COVID-19) in Wuhan, China, led to global health concerns.
  • The causative agent, SARS-CoV-2, was identified, prompting efforts to isolate the virus from affected individuals worldwide.

Purpose of the Study:

  • To isolate and identify the SARS-CoV-2 virus from patients in South Korea presenting with pneumonia.
  • To characterize the isolated virus through genomic sequencing and electron microscopy.

Main Methods:

  • Respiratory samples from suspected COVID-19 patients were used for virus isolation in cell cultures.
  • Full genome sequencing was performed to determine viral genetic relatedness.
  • Electron microscopy was employed to visualize viral morphology.

Main Results:

  • The virus replicated successfully in Vero cells, showing cytopathic effects.
  • Genomic sequencing confirmed the isolated virus as SARS-CoV-2, with over 99.9% homology to strains from China.
  • Electron microscopy revealed characteristic coronavirus morphology.

Conclusions:

  • SARS-CoV-2 was successfully isolated from South Korean patients with COVID-19 symptoms.
  • The isolated strain, BetaCoV/Korea/KCDC03/2020, is genetically similar to other SARS-CoV-2 isolates.
  • This isolation is crucial for further research and development of diagnostic and therapeutic strategies.
Abstract

Keywords:
COVID-19SARS-CoV-2coronavirusisolationpneumonia

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