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Related Concept Videos

Asepsis01:28

Asepsis

2.8K
The condition of being free from disease-causing living pathogens is asepsis. Aseptic techniques include a set of standard practices to achieve asepsis. An example is the regular environmental cleaning of all parts of the healthcare facility and hand hygiene at home before preparing or eating food. Medical and surgical asepsis in healthcare practice protects patients from harmful pathogens, minimizes the risk of contamination of susceptible sites, and reduces the risk of infection transmission.
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Key Techniques in Microbiology01:19

Key Techniques in Microbiology

1.3K
Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
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Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

1.2K
Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
1.2K
Hand hygiene01:23

Hand hygiene

5.4K
Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...
5.4K
Tracheostomy Suctioning II: Procedure01:23

Tracheostomy Suctioning II: Procedure

1.3K
Tracheostomy suctioning is a vital nursing procedure that involves removing secretions from the tracheostomy tube to maintain airway patency and prevent respiratory complications. Nurses need to understand the proper technique for tracheostomy suctioning to ensure patient safety and comfort. In this guide, we will outline the step-by-step process for performing tracheostomy suctioning, including preparing the sterile field, donning personal protective equipment (PPE), lubricating and connecting...
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Differential Staining Technique01:26

Differential Staining Technique

1.6K
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
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Updated: Dec 26, 2025

Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
11:43

Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors

Published on: May 31, 2012

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Aseptic Technique.

Tomasz Bykowski1, Brian Stevenson2

  • 1Center for Medical Education, Warsaw, Poland.

Current Protocols in Microbiology
|March 10, 2020
PubMed
Summary
This summary is machine-generated.

Learn essential aseptic techniques to prevent laboratory culture contamination (sepsis). This guide details Bunsen burner and laminar flow hood methods for common microbiology tasks, ensuring sterile procedures.

Keywords:
Bunsen burneraseptic techniquebacteriologycell culturelaminar flowlaminar hoodsterilizationvirology

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Aseptic Laboratory Techniques: Plating Methods
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Area of Science:

  • Microbiology
  • Laboratory Science

Background:

  • Culture contamination, or sepsis, poses a significant risk in laboratory settings.
  • Maintaining sterile conditions is crucial for accurate experimental results.

Purpose of the Study:

  • To describe common laboratory procedures that minimize the risk of culture contamination.
  • To provide general protocols for aseptic technique applicable to various laboratory tasks.

Main Methods:

  • Utilizing a Bunsen burner for aseptic work.
  • Employing a laminar flow hood for sterile procedures.
  • General protocols cover pipetting, media preparation, and microbial inoculation/culturing.

Main Results:

  • Standardized protocols for aseptic technique were presented.
  • Two primary methods (Bunsen burner, laminar flow hood) were detailed.
  • Applicability across diverse tasks like media preparation and microbial handling was demonstrated.

Conclusions:

  • Aseptic technique is vital for preventing culture contamination.
  • Bunsen burners and laminar flow hoods are effective tools for sterile laboratory work.
  • These methods support reliable microbial research and diagnostics.