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Related Concept Videos

Voltammetric Techniques: Pulse Voltammetry01:17

Voltammetric Techniques: Pulse Voltammetry

Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...

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Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers
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Salivary Cortisol Determination on Smartphone-Based Differential Pulse Voltammetry System.

Jingjing Liu1,2,3, Ning Xu2, Hong Men2

  • 1Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China.

Sensors (Basel, Switzerland)
|March 11, 2020
PubMed
Summary

This study presents a novel smartphone-based immunosensor for detecting cortisol, a key stress biomarker, in saliva. The developed device offers a sensitive and practical approach for non-invasive personal health monitoring at the point of care.

Keywords:
differential pulse voltammetrygold nanoparticlesimmunosensormolybdenum disulfidesalivary cortisolsmartphone

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Cortisol is a crucial biomarker for assessing psychological and physical stress.
  • Non-invasive, point-of-care (POC) cortisol detection is increasingly vital for personal health monitoring.
  • Existing methods may lack the sensitivity or convenience required for widespread POC application.

Purpose of the Study:

  • To develop an ultrasensitive immunosensor for non-invasive salivary cortisol detection at POC.
  • To integrate the immunosensor with a miniaturized, smartphone-based system for portable analysis.
  • To evaluate the performance and practicality of the developed system for real-world applications.

Main Methods:

  • Fabrication of a transducer using gold nanoparticles/molybdenum disulfide/gold nanoparticles (AuNPs/MoS2/AuNPs).
  • Covalent immobilization of cortisol antibody (CORT-Ab) via a polyethylene glycol (PEG) linker.
  • Utilizing a smartphone-based miniaturized differential pulse voltammetry (DPV) system for detection.
  • Surface passivation with ethanolamine to prevent non-specific binding.

Main Results:

  • The immunosensor demonstrated a linear detection range for cortisol from 0.5 to 200 nM.
  • Achieved a low detection limit of 0.11 nM and high sensitivity of 30 μA M-1 (R²=0.9947).
  • Successfully distinguished cortisol from other salivary components and showed good recovery rates for spiked samples.

Conclusions:

  • The smartphone-based immunosensor system exhibits excellent performance for salivary cortisol detection.
  • The developed system holds significant potential for non-invasive, POC personal health monitoring.
  • This technology offers a convenient and sensitive tool for stress level assessment.