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In vitro activity of isavuconazole against three species of Acanthamoeba

K Brunet1, R Eestermans2, M-H Rodier3

  • 1Laboratoire de parasitologie et mycologie médicale, CHU de Poitiers, bâtiment UBM, 2(e) étage, 2, rue de la Milétrie, 86021 Poitiers, France; Inserm U1070, université de Poitiers, Poitiers, France.

Journal Francais D'Ophtalmologie
|March 11, 2020
PubMed

Insights

Isavuconazole shows minimal effectiveness against Acanthamoeba keratitis pathogens. It may help manage infections caused by Acanthamoeba castellanii when used with other drugs.

Area of Science:

  • Ophthalmology
  • Infectious Diseases
  • Mycology

Background:

  • Acanthamoeba keratitis (AK) is a severe corneal infection.
  • Current AK treatments involve antiseptics and azole antifungals.
  • Newer antifungal agents require evaluation against resistant pathogens.

Purpose of the Study:

  • To assess the in vitro efficacy of isavuconazole against Acanthamoeba species.
  • To determine if isavuconazole has amoebistatic or amoebicidal activity.
  • To inform potential therapeutic strategies for Acanthamoeba keratitis.

Main Methods:

  • In vitro testing of isavuconazole.
  • Exposure of Acanthamoeba castellanii, A. lenticulata, and A. hatchetti to isavuconazole.
  • Evaluation of trophozoite and cysticidal activity.

Main Results:

  • Isavuconazole demonstrated slight amoebistatic activity against Acanthamoeba castellanii trophozoites.
  • No significant cysticidal activity was observed for isavuconazole.
  • Variable efficacy was noted across different Acanthamoeba species tested.

Conclusions:

  • Isavuconazole exhibits limited direct efficacy against Acanthamoeba keratitis pathogens.
  • Combination therapy including isavuconazole may be necessary for managing A. castellanii infections.
  • Further research is needed to explore synergistic treatment options for AK.
Keywords:
AcanthamoebaAmibe libreFree-living amoebaeIsavuconazoleKeratitisKératite

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