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CircCBFB-mediated miR-28-5p facilitates abdominal aortic aneurysm via LYPD3 and GRIA4

Jianing Yue1, Ting Zhu1, Jue Yang1

  • 1Department of Vascular Surgery, Zhongshan Hospital, Fudan University, No.180 Fenglin Road, Xuhui District, Shanghai 200032, China.

Life Sciences
|March 11, 2020
PubMed
Abstract

Insights

This study reveals that the circCBFB/miR-28-5p/GRIA4/LYPD3 axis significantly impacts vascular smooth muscle cell apoptosis, offering new insights for abdominal aortic aneurysm management.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Vascular Biology

Background:

  • Abdominal aortic aneurysm (AAA) is characterized by vascular smooth muscle cell (VSMC) growth impairment and apoptosis.
  • MicroRNAs (miRNAs) play a crucial role in regulating cellular processes like growth and apoptosis.
  • miR-28-5p has been implicated in AAA pathogenesis.

Purpose of the Study:

  • To investigate the role of miR-28-5p in VSMC apoptosis within the context of AAA.
  • To identify downstream targets and regulatory mechanisms of miR-28-5p in AAA.

Main Methods:

  • Functional assays to assess VSMC apoptosis.
  • Bioinformatic analyses to identify downstream mRNA targets of miR-28-5p.
  • Investigation of interactions between miR-28-5p, GRIA4, LYPD3, and circCBFB.
  • Rescue experiments to validate the identified molecular axis.

Main Results:

  • miR-28-5p promotes VSMC apoptosis, while circCBFB, GRIA4, and LYPD3 exhibit anti-apoptotic effects.
  • miR-28-5p directly suppresses GRIA4 and LYPD3 expression.
  • circCBFB acts as a molecular sponge for miR-28-5p, thereby releasing GRIA4 and LYPD3.
  • The circCBFB/miR-28-5p/GRIA4/LYPD3 axis is essential for regulating VSMC apoptosis.

Conclusions:

  • A novel circCBFB/miR-28-5p/GRIA4/LYPD3 axis regulating VSMC apoptosis has been elucidated.
  • This axis presents a potential therapeutic target for managing abdominal aortic aneurysm.
Keywords:
AAAGRIA4LYPD3VSMC apoptosiscircCBFBmiR-28-5p

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