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Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

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Published on: June 7, 2020

A progesterone biosensor derived from microbial screening.

Chloé Grazon^1,2, R C Baer^3,4, Uroš Kuzmanović⁵

¹Department of Chemistry, Boston University, Boston, MA, 02215, USA.

Nature Communications

|March 11, 2020

Summary

This summary is machine-generated.

Researchers developed a novel method to find bacterial allosteric transcription factors (aTFs) for creating biosensors. They successfully created an optical sensor for progesterone detection, suitable for point-of-care use.

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Area of Science:

Microbiology
Biotechnology
Molecular Biology

Background:

Bacteria offer a vast, underexplored source of proteins for biosensing applications.
Allosteric transcription factors (aTFs) are key regulatory proteins that can be engineered for analyte recognition.

Purpose of the Study:

To develop a method for identifying and isolating bacterial aTFs for biosensor development.
To create a functional optical biosensor for progesterone detection using a bacterial aTF.

Main Methods:

Genomic screening and functional assays were employed to identify and isolate specific aTFs.
Quantum-dot Förster Resonance Energy Transfer (FRET) was utilized for real-time, quantitative signal transduction.
A progesterone-sensing aTF was identified and engineered into an optical sensor.

Main Results:

The developed biosensor accurately detects progesterone in artificial urine.
The sensor demonstrates sufficient sensitivity and specificity for potential clinical applications.
The system is compatible with inexpensive, portable electronic readers for point-of-care diagnostics.

Conclusions:

This study presents a proof-of-concept for microbially-derived biosensors.
The approach is adaptable for creating inexpensive, real-time sensor devices for various analytes.