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Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi
Jisoo Yoo1, Hankyul Kwak2, Juyoung Kwon1
1Department of Materials Science and Engineering, Yonsei University, Seoul, 03722, Republic of Korea.
Scientific Reports
|March 11, 2020
Summary
This study introduces a novel method for long-term intracellular recording in single cells using vertical nanowire multi electrode arrays (VNMEA) and optogenetics. This technique enables continuous, minimally disruptive monitoring of cellular activity over 24 hours.
Area of Science:
- Biophysics
- Cell Biology
- Neuroscience
Background:
- Continuous intracellular recording is crucial for understanding rare, dynamic, and heterogeneous cell responses.
- Existing methods face technical challenges in achieving long-term, minimally disruptive single-cell measurements.
Purpose of the Study:
- To develop a method for long-term, minimally disruptive intracellular recording in single cells.
- To integrate vertical nanowire multi electrode arrays (VNMEA) with optogenetic stimulation for enhanced cellular analysis.
Main Methods:
- Synthesized small-diameter, high-aspect-ratio silicon nanowires for spontaneous cell penetration.
- Utilized optogenetic stimulation to modulate cell responsiveness.
- Simultaneously measured light-induced intra- and extracellular activities of optogenetically-modified cells.
Main Results:
- Achieved continuous and reliable intracellular recordings over 24 hours without signal interference or attenuation.
- Demonstrated distinct measurement characteristics based on cell-electrode configuration.
- Validated the minimally disruptive nature of the VNMEA approach for cell survival and function.
Conclusions:
- The integration of VNMEA and optogenetics offers a powerful strategy for long-term intracellular recording.
- This technique expands possibilities for discovering mechanisms of physiological and dynamic cellular processes.
- Enables detailed study of individual cell behaviors crucial for various research fields.
