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A single digestion, single-stranded oligonucleotide mediated PCR-independent site-directed mutagenesis method

Mengjie Dong1, Fei Wang1, Qingqing Li1

  • 1State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, School of Life Sciences, Hubei University, Wuhan, People's Republic of China.

Applied Microbiology and Biotechnology
|March 11, 2020
PubMed

Keywords:
FnCas12aSingle digestionSingle-stranded oligonucleotideSite-directed mutagenesisT5 exonuclease

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