Related Experiment Video

Updated: Dec 26, 2025

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
07:33

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Published on: December 19, 2020

7.0K

Action of antimicrobial peptides and cell-penetrating peptides on membrane potential revealed by the single GUV

Md Mizanur Rahman Moghal1,2, Farzana Hossain1, Masahito Yamazaki3,4,5

  • 1Integrated Bioscience Section, Graduate School of Science and Technology, Shizuoka University, Shizuoka, 422-8529, Japan.

Biophysical Reviews
|March 11, 2020
PubMed

Insights

Membrane potential significantly influences antimicrobial peptides (AMPs) and cell-penetrating peptides (CPPs). The single giant unilamellar vesicle (GUV) method reveals how membrane potential affects peptide interactions with membranes.

Area of Science:

  • Biophysics
  • Cell Biology
  • Membrane Science

Background:

  • Membrane potential is crucial for cellular functions in bacteria and eukaryotes.
  • Previous studies on antimicrobial peptides (AMPs) and cell-penetrating peptides (CPPs) using cells and small vesicles faced technical limitations.
  • The precise effects of membrane potential on peptide-membrane interactions remain incompletely understood.

Purpose of the Study:

  • To review the impact of membrane potential on the action of lactoferricin B (LfcinB), an AMP, and transportan 10 (TP10), a CPP.
  • To elucidate the elementary steps of peptide-membrane interactions under varying membrane potentials.
  • To highlight the utility of the single giant unilamellar vesicle (GUV) method for studying these phenomena.

Main Methods:

  • Utilizing the single giant unilamellar vesicle (GUV) method to monitor peptide-membrane interactions.
  • Investigating the effects of membrane potential on vesicle membranes.
  • Analyzing the actions and elementary steps of AMPs and CPPs on GUVs.

Main Results:

  • The single GUV method clearly revealed the actions of AMPs and CPPs on vesicle membranes under membrane potential.
  • Specific steps of peptide-membrane interactions were elucidated.
  • Demonstrated the influence of membrane potential on peptide efficacy.

Conclusions:

  • The single GUV method provides a powerful tool to study peptide-membrane interactions influenced by membrane potential.
  • This approach can be extended to investigate various peptides, including AMPs, CPPs, and proteins.
  • GUVs with membrane potential serve as effective models for biological cells and artificial cell systems.
Keywords:
Antimicrobial peptidesArtificial cellsBio-imagingCell-penetrating peptidesMembrane potentialSingle giant unilamellar vesicles

More Related Videos

Measuring Peptide Translocation into Large Unilamellar Vesicles12:27

Measuring Peptide Translocation into Large Unilamellar Vesicles

Published on: January 27, 2012

14.2K
Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties10:52

Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties

Published on: October 13, 2010

13.2K

Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
6.2K