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Enzymatic amplification and characterization of large DNA fragments from genomic DNA
P Keohavong1, C C Wang, R S Cha
1MIT Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge 02139.
Gene
|November 15, 1988
Abstract:
Conditions for DNA amplification in vitro using modified T7 DNA polymerase have been devised to obtain 2000-bp DNA fragments of the HGPRT gene directly from human genomic DNA. The DNA obtained from a 1.2 x 10(5)-fold amplification has been used for direct sequencing.