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A Cyclodextrin-Based Controlled Release System in the Simulation of In Vitro Small Intestine
Danni Zheng1,2,3, Liuxi Xia1,2,3,4, Hangyan Ji1,2,3
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Molecules (Basel, Switzerland)
|March 12, 2020
Summary
A novel cyclodextrin system controls drug release in the small intestine using enzymes. Varying cyclodextrin glycosyltransferase levels precisely regulate guest molecule release for targeted drug delivery.
Area of Science:
- Pharmaceutical Sciences
- Biotechnology
- Materials Science
Background:
- Controlled drug release systems are crucial for optimizing therapeutic efficacy and patient compliance.
- Cyclodextrins (CDs) are versatile host molecules for forming inclusion complexes with various drugs.
- Developing targeted delivery systems for the small intestine remains a significant challenge in pharmaceutical research.
Purpose of the Study:
- To develop a novel cyclodextrin (CD)-based controlled release system for the small intestine.
- To utilize endogenous enzymes in the small intestine for triggering drug release.
- To precisely control the rate of guest molecule release from CD complexes.
Main Methods:
- Preparation and characterization of vanillin/curcumin-β-CD complexes using FT-IR, TGA, and DSC.
- Enzymatic degradation of host CD using exogenous β-cyclodextrin glycosyltransferase (β-CGTase) and endogenous amyloglucosidase (AG).
- Monitoring guest release by measuring glucose production and color disappearance.
Main Results:
- The vanillin/curcumin-β-CD complexes were successfully prepared and characterized.
- The combination of β-CGTase and AG effectively degraded the host CD and released guest molecules.
- Varying β-CGTase concentration allowed precise control over the rate of CD degradation and guest release.
- In vitro simulations confirmed the feasibility of the controlled release system in the small intestine.
Conclusions:
- A novel enzymatic, CD-based controlled release system was successfully developed for the small intestine.
- The system leverages endogenous enzymes (MG/AG) and exogenous β-CGTase for tunable drug release.
- This approach offers potential for advanced drug delivery and absorption strategies in the small intestine.
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