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Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
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A Surface-Tailoring Method for Rapid Non-Thermosensitive Cell-Sheet Engineering via Functional Polymer Coatings.

Jieung Baek1, Younghak Cho1, Hyun-Ji Park2

  • 1Department of Chemical and Biomolecular Engineering and KI for NanoCentury, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.

Advanced Materials (Deerfield Beach, Fla.)
|March 12, 2020
PubMed
Summary

This study introduces a rapid, non-thermoresponsive method for cell sheet engineering, enabling quick harvesting of cell sheets for regenerative medicine applications without artificial biomaterials.

Keywords:
cell sheetsdivalent cation depletioninitiated chemical vapor deposition (iCVD)surface energy modulationtissue regeneration

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Cell sheet engineering offers a scaffold-free approach for tissue regeneration.
  • Conventional methods often require artificial biomaterials for cell sheet harvesting and transplantation.
  • Existing techniques may involve thermal stimuli, limiting applications.

Purpose of the Study:

  • To develop a facile and rapid non-thermoresponsive method for cell sheet engineering.
  • To create a reliable platform for prompt cell sheet harvesting.
  • To demonstrate the therapeutic potential of engineered cell sheets in vivo.

Main Methods:

  • Utilized functional polymer coatings to precisely modulate cell-substrate interactions and surface energy.
  • Engineered a surface that triggers spontaneous cell sheet detachment upon depletion of divalent cations.
  • Achieved prompt cell sheet harvesting within 100 seconds under physiological conditions.

Main Results:

  • Demonstrated a rapid (within 100 s) and reliable cell sheet harvesting platform.
  • Successfully detached cell sheets under physiological conditions (pH 7.4, 37 °C) without thermal stimuli.
  • Showcased therapeutic efficacy by transplanting multilayered cell sheets into mouse models of diabetic wounds and ischemia.

Conclusions:

  • The developed surface provides a robust platform for non-thermoresponsive cell sheet engineering.
  • This technique represents a significant breakthrough for regenerative medicine applications.
  • The method facilitates cell transplantation without artificial biomaterials, enhancing its clinical potential.