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Dynamic Environmental Control in Microfluidic Single-Cell Cultivations: From Concepts to Applications
Sarah Täuber1, Eric von Lieres2, Alexander Grünberger1
1Multiscale Bioengineering, Faculty of Technology, Bielefeld University, Universitätsstraße 25, 33615, Bielefeld, Germany.
Small (Weinheim an Der Bergstrasse, Germany)
|March 12, 2020
Summary
Microfluidic single-cell cultivation (MSCC) now explores dynamic environments, moving beyond constant conditions. This research details microfluidic methods for dynamic MSCC, enabling new insights into cellular behavior under fluctuating conditions.
Area of Science:
- Biotechnology
- Cell Biology
- Microfluidics
Background:
- Microfluidic single-cell cultivation (MSCC) traditionally uses constant environmental conditions.
- Recent interest is growing in MSCC under oscillating and dynamic conditions to study cellular responses.
- Investigating cellular behavior in fluctuating environments is crucial for understanding biological systems.
Purpose of the Study:
- To provide an overview of microfluidic single-cell cultivation under dynamic environmental conditions.
- To discuss microfluidic concepts for controlling dynamic medium conditions in MSCC.
- To demonstrate applications of dynamic MSCC in mammalian and microbial cell systems.
Main Methods:
- Review of microfluidic concepts enabling dynamic control of environmental parameters (e.g., medium composition, pH, O2).
- Demonstration of MSCC systems for dynamic cultivation of mammalian and microbial cells.
- Discussion of technical progress in creating complex dynamic environmental profiles.
Main Results:
- Microfluidic systems can effectively control dynamic environmental conditions for single-cell cultivation.
- Dynamic MSCC is applicable to both mammalian and microbial cell systems.
- Technical advancements facilitate complex, multi-parameter dynamic environmental control.
Conclusions:
- Dynamic MSCC offers novel experimental approaches for single-cell research.
- This field lays the foundation for systematic analysis of cellular metabolism in fluctuating environments.
- Future work will focus on complex dynamic profiles for deeper biological insights.
