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Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge

Zhanfeng Yang1, Jingjing Zhang1, Danghui Lu2

  • 11Department of Medicine, Zhengzhou University of Industry Technology, 16 Xueyuan Road, Xinzheng, 451100 Henan China.

Cancer Cell International
|March 12, 2020
PubMed
Abstract

Insights

Overexpression of circular RNA hsa_circ_0137008 inhibits colorectal cancer (CRC) progression by sponging microRNA-338-5p. This hsa_circ_0137008/miR-338-5p axis acts as a key regulator in CRC tumorigenesis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in cancer development.
  • The specific role of hsa_circ_0137008 in colorectal cancer (CRC) tumorigenesis requires elucidation.

Purpose of the Study:

  • To investigate the function of hsa_circ_0137008 in colorectal cancer (CRC).
  • To explore the molecular mechanism underlying hsa_circ_0137008's role in CRC.

Main Methods:

  • Quantitative real-time PCR and Western blot for gene expression analysis.
  • In vitro assays (cell count, colony formation, wound healing, Transwell) to assess cell proliferation, viability, migration, and invasion.
  • Luciferase reporter and RNA pull-down assays to determine molecular interactions.
  • In vivo xenograft tumor model to evaluate the role of hsa_circ_0137008.

Main Results:

  • hsa_circ_0137008 was downregulated in CRC tissues and cell lines.
  • Overexpression of hsa_circ_0137008 suppressed CRC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT).
  • hsa_circ_0137008 acts as a sponge for microRNA-338-5p (miR-338-5p), inhibiting its pro-tumorigenic effects in CRC.

Conclusions:

  • Overexpression of hsa_circ_0137008 inhibits CRC progression by sponging miR-338-5p.
  • The hsa_circ_0137008/miR-338-5p axis is a critical regulator of colorectal cancer tumorigenesis.
Keywords:
Colorectal cancerHsa_circ_0137008InvasionMigrationProliferationmicroRNA-338-5p

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