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Cell Lines

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...

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Related Experiment Video

Updated: Dec 26, 2025

An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines

An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines

Published on: October 11, 2022

Tick Cell Lines in Research on Tick Control.

Ahmed Al-Rofaai¹, Lesley Bell-Sakyi¹

¹Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom.

Frontiers in Physiology

|March 12, 2020

Summary

Tick cell lines offer a vital in vitro tool for studying acaricide resistance and developing novel control strategies against these economically important disease vectors. This research aids in finding new ways to combat tick-borne diseases in animals and humans.

Keywords:

Ixodes spp.Rhipicephalus microplus acaricide anti-tick vaccine control metabolism resistance tick cell line

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Last Updated: Dec 26, 2025

An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines

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Published on: October 11, 2022

RNA Interference in Ticks

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Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research

Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research

Published on: August 31, 2013

Area of Science:

Veterinary Entomology
Parasitology
Molecular Biology

Background:

Ticks transmit diseases of significant global veterinary, medical, and economic impact.
Acaricide resistance in ticks necessitates continuous research for novel control methods.
Tick cell lines provide valuable in vitro models for tick research.

Purpose of the Study:

To review the utility of tick cell lines in advancing acaricide resistance research.
To explore the role of tick cell lines in understanding acaricide mechanisms and identifying new control targets.
To highlight the contribution of tick cell lines to anti-tick vaccine development.

Main Methods:

Utilizing continuous tick cell lines derived from susceptible and resistant tick populations.
Employing genetically modified tick cells and cell sub-lines with in vitro-generated resistance.
Conducting in vitro studies as an ethical and cost-effective alternative to in vivo experiments.

Main Results:

Tick cell lines facilitate detailed cellular and molecular investigations into acaricide resistance.
These cell lines aid in elucidating acaricide modes of action and tick metabolism.
Tick cell line research supports the development of novel anti-tick strategies and vaccines.

Conclusions:

Tick cell lines are indispensable tools for studying tick biology and developing innovative control measures.
Their application accelerates the search for new acaricides and anti-tick vaccines.
Continued use of tick cell lines will be crucial for managing tick-borne diseases.